For research use only (RUO). Not intended for human or veterinary use.
What does "lyophilized" mean?
Lyophilized peptides are freeze-dried to remove moisture, extending shelf life and improving stability during shipping. Before use in research, they must be reconstituted — dissolved back into liquid form using an appropriate solution.
What solution should I use to reconstitute lyophilized peptides?
The most commonly used solution is bacteriostatic water (BAC Water) — sterile water containing 0.9% benzyl alcohol as a preservative. BAC Water inhibits microbial growth, extending the usable life of your reconstituted peptide solution, which makes it the preferred choice over plain sterile water for most peptide research protocols.
Insulfine offers BAC Water in both 10 mL and 30 mL formats to suit different research volumes.
See also: Reconstitution Solution vs Sterile Water: Which Is Right for Your Research?
How much BAC Water do I need?
The volume depends on your target concentration. A common starting point is 1 mL of BAC Water per 1 mg of peptide, giving a 1 mg/mL working solution. For a 5 mg vial, adding 2 mL gives a 2.5 mg/mL concentration — adjust based on your protocol.
If you're running multiple reconstitutions, a larger pack like the 10 mL 9-Pack or 30 mL 4-Pack will keep you stocked without frequent reorders.
See: How Much BAC Water Do You Need? A Simple Guide
What's the correct reconstitution technique?
- Allow the vial to reach room temperature before opening
- Add BAC Water slowly along the side of the vial — do not inject directly onto the powder
- Gently swirl; do not shake or vortex
- Allow to dissolve fully (1–5 minutes depending on the peptide)
- Inspect for clarity before use
Full step-by-step protocol: How to Reconstitute Peptides: A Step-by-Step Guide
What are the most common reconstitution mistakes?
The biggest errors researchers make include shaking the vial vigorously (which degrades the peptide), using non-sterile diluents, injecting the solution directly onto the lyophilized cake, and skipping temperature equilibration. Using the wrong buffer for pH-sensitive peptides is another frequent issue that affects yield and stability.
Full breakdown: Common Mistakes When Reconstituting Peptides (And How to Avoid Them)
How should I store reconstituted peptides?
For short-term use (up to four weeks), refrigerate at 2–8°C. For longer storage, aliquot into single-use volumes and freeze at -20°C or -80°C — repeated freeze-thaw cycles are one of the fastest ways to degrade peptide integrity.
Detailed stability guide: How to Store Reconstituted Peptides: Stability & Shelf Life Guide
Why does BAC Water contain benzyl alcohol?
Benzyl alcohol at 0.9% acts as a bacteriostatic preservative, preventing microbial growth without sterilizing the solution. This is especially important for multi-use vials in a lab setting where the same vial may be accessed multiple times over days or weeks.
Full explanation: Why Does BAC Water Contain Benzyl Alcohol?
What size vial should I choose — 10 mL or 30 mL?
It depends on your protocol volume. The 10 mL vials work well for lower-volume applications or when you're working with a single peptide at a time. The 30 mL vials are a better fit for labs running multiple reconstitutions or higher-concentration protocols, and they offer a lower cost per mL.
For bulk lab supply, the 30 mL 8-Pack and 30 mL 12-Pack are the most cost-efficient options.
Comparison guide: How to Choose 10 mL vs 30 mL BAC Water Packs
Does Insulfine provide a Certificate of Analysis (COA)?
Yes. Every batch of Insulfine BAC Water Reconstitution Solution ships with a COA confirming purity, sterility, and benzyl alcohol concentration — so you have documentation to support your research records.
What a COA Means for BAC Water Buyers
Is Insulfine BAC Water available for fast delivery in the US?
Yes — Insulfine ships from a US warehouse, which means consistent lead times and reliable availability for repeat research orders. No waiting on international shipping for time-sensitive protocols.
Why US Warehouse Availability Matters for Repeat Orders
All Insulfine products are for research use only (RUO). Not intended for human or veterinary use.